Pseudomonas paraeruginosa strain:MAZ105 Genome sequencing and assembly. Pseudomonas paraeruginosa strain:MAZ105

In recent years, atypical strains of Pseudomonas aeruginosa have been isolated. These strains do not have the type III secretion system (T3SS), but contain a novel the two-partner secretion system called ExlBA. These atypical strains have been isolated from different environments, some have clinical and another environmental origin. The MAZ105 strain was isolated from the tomato rhizosphere in the state of Guerrero, Mexico, being the first strain to belong to group 3 identified in this country. This shows that these atypical strains of P. aeruginosa, are distributed around the world, but their study is limited. In this study, we focused on studying the virulence factors regulated by Quorum sensing response, as well as analysis of the sequence of regulators transcriptional quorum sensing (LasR, RhlR and PqsR) in strains that belong to group 3 and advance in the study of these atypical strains for a future find effective therapeutic targets.The study of the group 3 strains to which the MAZ105 strain belongs, will allow a precise analysis of the virulence mechanisms and pathogenicity that present these strains. The strains that belong to the P. aeruginosa group 3 present mutations in transcriptional regulators of Quorum sensing: LasR and PqsR, which are the main therapeutic targets for which have developed antivirulence compounds. However in strains belonging to this group, they won-t have effect. The analysis of the strains belonging to this group, can allow the development of new therapeutic approaches that have a greater scope in all strains of P. aeruginosa and not be limited to only some strains.

近年来，科研人员已分离得到多株非典型铜绿假单胞菌（Pseudomonas aeruginosa）。此类菌株不携带III型分泌系统（type III secretion system, T3SS），但拥有一套命名为ExlBA的新型双伴侣分泌系统（two-partner secretion system）。此类非典型菌株已从多种环境中分离得到，部分分离自临床样本，其余则来自环境样本。
MAZ105菌株分离自墨西哥格雷罗州的番茄根际，是该国首个被鉴定的第3群铜绿假单胞菌菌株。
这表明此类非典型铜绿假单胞菌菌株广泛分布于全球，但相关研究仍较为有限。本研究聚焦于受群体感应（Quorum sensing）响应调控的毒力因子，同时对第3群菌株的群体感应转录调控因子（LasR、RhlR与PqsR）的序列展开分析，以期推进此类非典型菌株的研究，为未来筛选高效治疗靶点奠定基础。
对包含MAZ105菌株在内的第3群菌株开展研究，可精准解析此类菌株的毒力机制与致病性。第3群铜绿假单胞菌菌株的群体感应转录调控因子LasR与PqsR存在突变，而这两类因子正是当前抗毒力化合物的主要研发靶点。但针对此类菌株，现有抗毒力化合物往往无法发挥效用。对该类菌株的分析，有助于开发适用范围更广的新型治疗策略，而非仅局限于部分特定铜绿假单胞菌菌株。