Investigation of human peripheral T cells responsive to SARS-CoV-2 antigens

To elucidate the T cell epitopes of SARS-CoV-2, we stimulated human PBMCs from healthy donors and convalescent COVID-19 patients with various SARS-CoV-2 antigens, sorted the activated T cells and performed sc-RNA and -TCR sequencing. We obtained thousands of T cell clonotypes that responded to SARS-CoV-2 antigens, and identified the epitopes and restricting HLAs of several clonotypes that were significantly expanded in the COVID-19 patients. Determination of the TCR and mRNA expression of human T cells stimulated with SARS-CoV-2 antigens.

为阐明新型冠状病毒（SARS-CoV-2）的T细胞表位，我们使用多种SARS-CoV-2抗原刺激健康供者与新型冠状病毒肺炎（COVID-19）康复患者的人外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs），随后分选活化T细胞并开展单细胞RNA（sc-RNA）与T细胞受体（TCR）测序。本研究共获得数千种可响应SARS-CoV-2抗原的T细胞克隆型，并鉴定出在COVID-19康复患者体内显著扩增的数种克隆型所对应的表位及其限制性人类白细胞抗原（HLAs）。本数据集包含经SARS-CoV-2抗原刺激的人T细胞的TCR及mRNA表达检测数据。