Effect of interleukin-10 on primary oligodendrocyte progenitor cells under hemin administration

To investigate the gene expression changes in primary oligodendrocyte progenitor cells generated from wild-type mouse pups after hemin or hemin+interleukin-10 or hemin+interleukin-10+stattic treatment Ttreat wild-type mouse primary oligodendrocyte progenitor cells with veh or hemin (50 μM) or hemin (50 μM)+interleukin-10 (10 ng/mL) or hemin (50 μM)+interleukin-10 (10 ng/mL)+stattic (2.5 μM) for 12 h

为探究野生型小鼠幼鼠来源的原代少突胶质细胞前体细胞（primary oligodendrocyte progenitor cells）经血红素、血红素联合白细胞介素-10（interleukin-10），或血红素联合白细胞介素-10联合stattic处理后的基因表达变化，本实验采用溶媒对照（vehicle, veh）、50 μM血红素、50 μM血红素+10 ng/mL白细胞介素-10、50 μM血红素+10 ng/mL白细胞介素-10+2.5 μM stattic四种处理方案，对野生型小鼠原代少突胶质细胞前体细胞干预12小时。