miRNA expression profile in breast cancer cells. miRNA expression profile in breast cancer cells

We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from ZR75.1 cells expressing or not human ER-beta in absence of estrogen stimuli was subjected to size selection library preparation and retrotranscribed. Obtained DNA was sequenced with the Illumina HiSeq 1500 sequencer .

我们参照Illumina测序流程开展了微小RNA测序（microRNA sequencing, miRNA-seq）：从经或不表达人雌激素受体β（ER-beta）、且处于无雌激素刺激环境下的ZR75.1细胞中提取总RNA，随后对该总RNA进行片段选择文库制备与反转录；最终获得的DNA通过Illumina HiSeq 1500测序仪完成测序。