Table_3_Interaction Between Chronic Endometritis Caused Endometrial Microbiota Disorder and Endometrial Immune Environment Change in Recurrent Implantation Failure.xlsx

ObjectiveTo investigate the Interaction between chronic endometritis (CE) caused endometrial microbiota disorder and endometrial immune environment change in recurrent implantation failure (RIF).
MethodTranscriptome sequencing analysis of the endometrial of 112 patients was preform by using High-Throughput Sequencing. The endometrial microbiota of 43 patients was analyzed by using 16s rRNA sequencing technology.
ResultIn host endometrium, CD4 T cell and macrophage exhibited significant differences abundance between CE and non-CE patients. The enrichment analysis indicated differentially expressed genes mainly enriched in immune-related functional terms. Phyllobacterium and Sphingomonas were significantly high infiltration in CE patients, and active in pathways related to carbohydrate metabolism and/or fat metabolism. The increased synthesis of lipopolysaccharide, an important immunomodulator, was the result of microbial disorders in the endometrium.
ConclusionThe composition of endometrial microorganisms in CE and non-CE patients were significantly different. Phyllobacterium and Sphingomonas mainly regulated immune cells by interfering with the process of carbohydrate metabolism and/or fat metabolism in the endometrium. CE endometrial microorganisms might regulate Th17 response and the ratio of Th1 to Th17 through lipopolysaccharide (LPS).

研究目的：探讨复发性植入失败（recurrent implantation failure, RIF）患者中，慢性子宫内膜炎（chronic endometritis, CE）引发的子宫内膜菌群紊乱与子宫内膜免疫微环境改变之间的相互作用。
研究方法：采用高通量测序（High-Throughput Sequencing）技术对112例患者的子宫内膜组织开展转录组测序分析；采用16S rRNA测序技术对43例患者的子宫内膜菌群进行检测分析。
研究结果：在宿主子宫内膜中，CD4 T细胞与巨噬细胞的丰度在CE患者与非CE患者间存在显著差异。富集分析显示，差异表达基因主要富集于免疫相关功能条目。叶杆菌属（Phyllobacterium）与鞘氨醇单胞菌属（Sphingomonas）在CE患者子宫内膜中显著高浸润，且其活性与碳水化合物代谢及/或脂肪代谢通路相关。脂多糖（lipopolysaccharide, LPS）作为重要的免疫调节因子，其合成增加是子宫内膜菌群紊乱的结果。
研究结论：CE患者与非CE患者的子宫内膜微生物组成存在显著差异。叶杆菌属与鞘氨醇单胞菌属主要通过干扰子宫内膜内的碳水化合物代谢及/或脂肪代谢过程来调控免疫细胞。CE患者的子宫内膜微生物或可通过脂多糖（LPS）调控Th17细胞应答以及Th1与Th17的细胞比值。