Biomarkers for Down Syndrome screening in murine fetal liver and placenta

The Ts1Cje mouse strain (Sago, 1998) contains a segmental trisomy of mouse chromosome 16 orthologous to the region of human chromosome 21 commonly associated with Down Syndrome. In this study, fetuses were obtained from wildtype mothers bred with either wildtype or Ts1Cje males. Gene expression profiles in fetal liver and placenta of wildtype and Ts1Cje fetuses were compared, to identify potential markers for application in human prenatal DS screening. A 48 array study with 24 arrays for placenta and 24 for fetal liver. For each tissue we used RNA from 24 individual embryos, i.e. six male and six female embryos from both genotypes.

Ts1Cje小鼠品系（Sago，1998）携带有小鼠16号染色体的一段片段性三体，该区域与人类21号染色体中常与唐氏综合征（Down Syndrome）相关的区段同源。本研究中，野生型母鼠分别与野生型或Ts1Cje公鼠交配以获取胎鼠。随后研究人员对野生型与Ts1Cje胎鼠的胎肝及胎盘的基因表达谱进行对比分析，以期筛选可用于人类产前唐氏综合征筛查的潜在标志物。本研究共采用48张基因芯片阵列开展检测，其中24张用于胎盘样本，24张用于胎肝样本。针对每种组织，实验均使用了24枚独立胚胎的RNA，即两种基因型各包含6枚雄性胚胎与6枚雌性胚胎。