Additional file 9: of A metagenomics roadmap to the uncultured genome diversity in hypersaline soda lake sediments

Dataset 6. Sheet 1 shows the MAGs positive for the marker gene acsB (K14138) encoding an acetyl-CoA synthase (ACS). The basis for Fig. 6, namely presence and absence of key genes involved in the Wood-Ljungdahly pathway, acetogenesis, methanogenesis, glycolysis and pyruvate to CO2 conversion is shown for each MAG. Sheet 2 shows the MAGs positive for the marker gene cdhC (K00193) encoding for the beta subunit of an acetyl-CoA decarboxylase synthase complex. While acsB and cdhC correspond roughly to the Bacterial-type and Archaeal-type (methanogens) enzymes with the same function, we found few discrepancies between marker gene and genome phylogeny within the Methanomassiliicoccaceae and Chloroflexi. (XLSX 52 kb)

数据集6。工作表1展示了携带编码乙酰辅酶A合成酶（ACS，Acetyl-CoA Synthase）的标记基因acsB（K14138）的宏基因组组装基因组（Metagenome-Assembled Genome，以下简称MAGs）。图6的构建依据为各MAG中伍德-隆德哈尔途径（Wood-Ljungdahl pathway）、产乙酸作用、产甲烷作用、糖酵解及丙酮酸转化为二氧化碳过程所涉及关键基因的存在与缺失情况，相关信息已针对每个MAG逐一列明。工作表2展示了携带标记基因cdhC（K00193）的MAGs，该基因编码乙酰辅酶A脱羧酶合成酶复合物的β亚基。尽管acsB与cdhC大致分别对应功能相同的细菌型与古菌型（产甲烷古菌）酶，但我们在甲烷毛球菌科（Methanomassiliicoccaceae）与绿弯菌门（Chloroflexi）中发现了少量标记基因与基因组系统发育不一致的情况。（XLSX格式，52 KB）