Suplememtary Materials

Lai et al., Correlation Analysis Between IFIT2 and HLA Class II in Oral Cancer.Supplementary Table 1. Associations of IFIT2 and clinical variables in tumors from patients with oral cancer.Figure S1: There was no significant difference in the proliferation rate after <i>in vivo</i> inoculation. (A) Schematic illustrating the generation of the clonal sublines from CLA27cells. (B) Representative images of sublines after <i>in vivo</i> selection under phase contrast and fluorescence microscopy. Bars = 50 μm. (C) The proliferation rate was evaluated using the colony formation assay. Cells in logarithmic growth were harvested and reseeded (1,000 cells per 100-mm dish) in triplicate and incubated for 10 days. Colonies of more than 50 cells were counted after fixation in methanol and stained with 10% Giemsa solution.Figure S2: Flow chart of the microarray data analysis. ShCTRL: parental CAL27 cells expressing the lentiviral pGIPZ vector; shIFIT2-1, and -2: sublines of parental CAL27 cells expressing the lentiviral IFIT2-pGIPZ vector; shCTRL-T1 and T2: primary tumor clones derived from two shCTRL xenograft tissues; shIFIT2-T: primary tumor clone derived from shIFIT2 xenograft tissue; shIFIT2-M1 and -M2: different metastatic clones derived from two metastatic tumors derived from shIFIT2 cells after tail vein inoculation. Figure S3: mRNA levels of HLA class II molecules in HNC based on the sample type obtained from the TCGA database.Figure S3. mRNA levels of HLA class II molecules in HNC based on the sample type obtained from the TCGA database.

Lai等人的《口腔癌中IFIT2与II类人类白细胞抗原（HLA Class II）的相关性分析》。补充表1：口腔癌患者肿瘤组织中IFIT2与临床变量的关联。
图S1：体内（in vivo）接种后细胞增殖速率无显著差异。(A) 从CLA27细胞构建克隆亚系的示意图。(B) 体内筛选后的亚系在相差显微镜与荧光显微镜下的代表性成像，标尺为50 μm。(C) 采用集落形成实验检测增殖速率：收集对数生长期细胞，以每100mm培养皿1000个细胞的密度接种3个复孔，孵育10天后，经甲醇固定、10%吉姆萨（Giemsa）染色后，计数含50个以上细胞的集落数。
图S2：微阵列数据分析流程图。ShCTRL：表达慢病毒pGIPZ载体的亲本CAL27细胞；shIFIT2-1与shIFIT2-2：表达慢病毒IFIT2-pGIPZ载体的亲本CAL27细胞亚系；shCTRL-T1和shCTRL-T2：从两株shCTRL异种移植瘤组织中分离得到的原代肿瘤克隆；shIFIT2-T：从shIFIT2异种移植瘤组织中分离得到的原代肿瘤克隆；shIFIT2-M1与shIFIT2-M2：尾静脉接种shIFIT2细胞后，从两株转移瘤中分离得到的不同转移克隆。
图S3：基于癌症基因组图谱（The Cancer Genome Atlas, TCGA）数据库获取的样本类型，头颈部癌（Head and Neck Cancer, HNC）组织中II类人类白细胞抗原分子的mRNA表达水平。
图S3：基于癌症基因组图谱（The Cancer Genome Atlas, TCGA）数据库获取的样本类型，头颈部癌（Head and Neck Cancer, HNC）组织中II类人类白细胞抗原分子的mRNA表达水平。