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Data underlying the publication: Interfacial protein adsorption behavior can be connected across a wide range of timescales using the microfluidic EDGE (Edge-based droplet GEneration) tensiometer

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DataCite Commons2024-07-03 更新2024-07-03 收录
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# IntroductionIn the current study, we expand the use of our EDGE (Edge-based Droplet GEneration) device, and validate the findings with ADT (automated drop tensiometer) results at complementary timescales.Furthermore, we explore if the EDGE tensiometer could distinguish effects occurring at high whey protein concentrations (2.5 – 10 wt%).In this dataset, you can find an excel file with an overview of all dataset named "EDGEvsADT_WPI_20240516_v09_final.xlxs" (in the Analyzed_Data folder).You can also find the raw data in the Raw_Data folder with .txt files.Finally, in the Plotted_Data_Scripts folder you can find all processed data from the excel file in .txt extension together with a Matlab code to plot the data.<br># AbstractHypothesisOur hypothesis is that dynamic interfacial tension values as measured by the partitioned-Edge-based Droplet GEneration (EDGE) tensiometry can be connected to those obtained with classical techniques, such as the automated drop tensiometer (ADT), expanding the range of timescales towards very short ones.<br>ExperimentsOil-water and air-water interfaces are studied, with whey protein isolate solutions (WPI, 2.5 – 10 wt%) as the continuous phase.The dispersed phase consists of pure n-hexadecane or air. The EDGE tensiometer and ADT are used to measure the interfacial (surface) tension at various timescales.A comparative assessment is carried out to identify differences between protein concentrations as well as between oil-water and air-water interfaces.<br>FindingsThe EDGE tensiometer can measure at timescales down to a few milliseconds and up to around 10 seconds, while the ADT provides dynamic interfacial tension values after at least one second from droplet injection and typically is used to also cover hours.The interfacial tension values measured with both techniques exhibit overlap, implying that the techniques provide consistent and complementary information.Unlike the ADT, the EDGE tensiometer distinguishes differences in protein adsorption dynamics at protein concentrations as high as 10 wt% (which is the highest concentration tested) at both oil-water and air-water interfaces.<br># Measurements and data collectionMeasurements and data collection were done according to what is described in the paperWe used two main setups and equipment, namely ADT (Automated Drop Tensiometer) and EDGE (partitioned-Edge-based Droplet GEneration) for data collection

# 引言 在本研究中,我们拓展了基于边缘的液滴生成(Edge-based Droplet GEneration, EDGE)装置的应用范围,并通过互补时间尺度下的自动落滴张力仪(automated drop tensiometer, ADT)测试结果对研究发现进行验证。此外,本研究还探究了EDGE张力仪能否区分高乳清蛋白浓度(2.5~10 wt%)下出现的相关效应。 本数据集的Analyzed_Data文件夹中包含一份汇总所有数据集信息的Excel文件,文件名为"EDGEvsADT_WPI_20240516_v09_final.xlxs"。Raw_Data文件夹中则存储了所有原始数据,格式为.txt文本文件。Plotted_Data_Scripts文件夹中包含了从上述Excel文件导出的所有处理后数据(格式为.txt),以及用于绘制数据图表的Matlab代码。 # 摘要 ## 研究假设 本研究的假设为:通过分区式基于边缘的液滴生成(EDGE)张力法测得的动态界面张力值,可与经典技术(如自动落滴张力仪ADT)所得结果建立关联,从而将可测量的时间尺度拓展至极短区间。 ## 实验设计 本研究探究了油-水界面与气-水界面的相关特性,实验连续相采用乳清分离蛋白(whey protein isolate, WPI)溶液(浓度范围2.5~10 wt%),分散相则为正十六烷纯品或空气。分别使用EDGE张力仪与ADT在不同时间尺度下测量界面(表面)张力,并开展对比分析,以厘清不同蛋白浓度以及油-水、气-水界面之间的差异。 ## 研究结果 EDGE张力仪可测量的时间尺度低至数毫秒,最高可达约10秒;而ADT则可在液滴注入至少1秒后获取动态界面张力值,通常还可覆盖数小时的时间区间。两种技术测得的界面张力值存在重叠区间,表明二者可提供一致且互补的实验数据。与ADT不同的是,EDGE张力仪能够在10 wt%(本研究测试的最高浓度)的蛋白浓度下,区分油-水与气-水界面中蛋白吸附动力学的差异。 # 测量与数据采集 本研究的测量与数据采集流程遵循论文中描述的规范。实验主要采用两套设备开展数据采集,分别为自动落滴张力仪(Automated Drop Tensiometer, ADT)与分区式基于边缘的液滴生成(EDGE)装置。
提供机构:
4TU.ResearchData
创建时间:
2024-05-17
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