Sensitivities of pairwise SHAPE-dependent sequence alignments relative to accepted alignments.

SHAPE-only sequence alignments did not use sequence information. Accepted alignments are from the CRW [11]. Nucleobase identity-based alignment used the Needle algorithm [14] on the EMBOSS server [15] with default parameters. For predictions incorporating both SHAPE reactivities and nucleobase identity, sensitivities are given for both pairwise comparisons and for multiple sequence alignments (MSA) generated by T-Coffee [16]. Sensitivities of pairwise SHAPE-dependent sequence alignments relative to accepted alignments.仅采用SHAPE的序列比对未纳入序列信息。经认可的比对结果源自CRW[11]。基于核苷酸碱基同一性的序列比对，通过EMBOSS服务器[15]上的Needle算法[14]完成，参数均采用默认设置。对于同时整合SHAPE反应性与核苷酸碱基同一性的预测任务，我们分别给出了成对序列比对与由T-Coffee[16]生成的多序列比对（MSA）的灵敏度数值。 基于SHAPE的成对序列比对相对于经认可比对的灵敏度。