Supplementary Fig. 5c & 6b | Western blots of FUS and TARDBP in iPSC lysates

This item is part of the Figshare Project:
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution

From Data Availability Statement for the forthcoming paper in Nature Communications entitled:
Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS
"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.
[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."
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[Item specific description:]
This data belong to Supplementary Fig. 5 and 6.
Western blots in iPSC lines with various FUS and TARDBP M337V mutations for FUS and TDP-43 protein expression.
The assembled western blots are presented in the PPTX file.
The fluorescent signal were scanned on a Odyssey CLx scanner (LI-COR) and exported as Image Studio ZIP archives. More information about the Image Studio software can be obtained here: https://www.licorbio.com/image-studio
The raw images are given as TIF files, thumbnails as PNG, and aquisition meta information as TXT files within the exported archives.

本数据集隶属于Figshare项目：**单细胞分辨率下揭示FUS与TARDBP相关肌萎缩侧索硬化症（Amyotrophic Lateral Sclerosis, ALS）的早期线粒体功能障碍**

本内容来自一篇即将发表于《自然-通讯》（*Nature Communications*）的论文的数据可用性说明，该论文标题为《单细胞RNA测序揭示FUS与TARDBP相关肌萎缩侧索硬化症共有的运动神经元特异性早期线粒体功能障碍》。
"本研究产生的所有原始及处理后RNA测序数据已上传至NCBI基因表达综合数据库（Gene Expression Omnibus, GEO），收录编号为GSE226482。C9orf72相关肌萎缩侧索硬化症的批量RNA测序数据直接从该研究作者处获取。"

【本Figshare项目包含以下内容】：荧光免疫印迹扫描图、共聚焦显微镜原始成像文件、Opera Phenix高内涵成像系统分析文件、定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）数据集，以及Seahorse细胞外流量检测结果文件。

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【本数据集专属说明】：
本数据集对应补充图5与补充图6。
本数据集为携带不同FUS及TARDBP M337V突变的诱导多能干细胞（induced pluripotent stem cell, iPSC）系中FUS与TDP-43蛋白表达的免疫印迹实验结果。
拼接完成的免疫印迹图已保存于PPTX文件中。
荧光信号通过Odyssey CLx扫描仪（LI-COR公司）完成扫描，并以Image Studio ZIP压缩包格式导出。如需获取Image Studio软件的更多信息，请访问：https://www.licorbio.com/image-studio
导出的压缩包内，原始图像以TIF格式存储，缩略图以PNG格式存储，实验采集元信息以TXT格式存储。