Single Cell RNA-Seq of Ear Epithelial Cells from i) steady state C57BL6/N & Tcrd-GDL mice, ii) untreated and TPA-treated C57BL6/J mice, and iii) IL13Ra1-knockdown CD1 mice. Single Cell RNA-Seq of Ear Epithelial Cells from i) steady state C57BL6/N & Tcrd-GDL mice, ii) untreated and TPA-treated C57BL6/J mice, and iii) IL13Ra1-knockdown CD1 mice

This dataset contains three experiments, designed for interrogating the effect of DETC on epithelial keratinocytes: Dataset 1) steady state ear epithelial cells (live, CD45-) from C57BL6/N (n=3) and Tcrd-GDL (n=3), after DETC deletion. Dataset 2) untreated and topical TPA-treated ear epithelial cells (live, CD45-, Vg5-) from C57BL6/J (n=3 untreated ears, n=3 treated ears). Dataset 3) steady state ear epithelial cells (GFP+) from CD1 (n=6) mice at age p8 weeks, following transduction with IL13Ra1-shRNA-GFP at e9.5.

本数据集包含三项实验，旨在探究DETC（表皮树突状T细胞）对上皮角质细胞的调控效应。数据集1：取自C57BL6/N（n=3）与Tcrd-GDL（n=3）小鼠的稳态耳部上皮细胞（活细胞，CD45阴性），样本均经DETC缺失处理。数据集2：取自C57BL6/J小鼠的未经处理与局部涂抹TPA（十四烷酰佛波醇乙酸酯）处理的耳部上皮细胞（活细胞，CD45阴性、Vg5阴性），其中未处理组耳部样本3例，处理组耳部样本3例。数据集3：取自6只8周龄CD1小鼠的稳态耳部上皮细胞（绿色荧光蛋白（GFP）阳性），该类细胞于胚胎发育第9.5天时通过IL13Ra1-shRNA-GFP（白细胞介素13受体α1-短发夹RNA-绿色荧光蛋白）完成转导处理。