ChIP-Seq of 2 Salicylic Acid resistant canola lines with H3K4me3 and Pol2 compared to the mother line after induction with SA. ChIP-Seq of 2 Salicylic Acid resistant canola lines with H3K4me3 and Pol2 compared to the mother line after induction with SA

An isogenic canola line was repeatedly treated with Salicylic Acid and the most vital plants were selected for propagation. 2 SA resistant lines were produced. ChIP-Seq was performed on those two lines and the mother line 6 hours after SA treatment to check for different reactions. Antibodies against H3K4me3 and Pol2 were used to check for changes in epigenetic makeup and transcriptional response.

本研究以一株同源同基因甘蓝型油菜（canola）品系为实验材料，反复施加水杨酸（Salicylic Acid）处理，并筛选生长状态最佳的植株进行扩繁，最终获得2株水杨酸抗性株系。随后，对这2株抗性株系及母本品系，在水杨酸处理6小时后开展染色质免疫共沉淀测序（ChIP-Seq）实验，以检测其应激响应差异。实验中使用针对组蛋白H3赖氨酸4三甲基化（H3K4me3）与RNA聚合酶II（Pol2）的抗体，分别探究其表观遗传组成与转录响应的变化情况。