Abscission layer (AL) at the base of flower in rice using laser micro-dissection: NIL(qSH1) AL vs. Nipponbare AL, NIL(qSH1) AL vs. NIL(qSH1) upper region of AL, and NIL(qSH1) AL vs. NIL(qSH1) lower region of AL. Oryza sativa

Tissue-specific transcriptional profiling of the abscission layer (AL) at the base of young flower in rice using laser micro-dissection: NIL(qSH1) vs. Nipponbare. Overall design: We used two rice varieties, NIL(qSH1) and Nipponbare. NIL(qSH1) is a nearly isogenic line containing the seed shattering gene qSH1. Seed shattering is easy in NIL(qSH1), but it is not in Nipponbare. So, we used some stages of young flower in NIL(qSH1) and some in Nipponbare. Four regions: 1. abscission layer region of NIL(qSH1), 2. upper abscission region of NIL(qSH1), 3. lower abscission layer region of NIL(qSH1), and 4. abscission layer region of Nipponbare. Sample experiments: NIL(qSH1) AL vs. Nipponbare AL, NIL(qSH1) AL vs. NIL(qSH1) upper region of AL, and NIL(qSH1) AL vs. NIL(qSH1) lower region of AL.

基于激光显微切割（laser micro-dissection）技术的水稻幼花基部离层（abscission layer, AL）组织特异性转录组分析：近等基因系NIL(qSH1)与日本晴（Nipponbare）对比。总体实验设计：本研究选用两个水稻品种，分别为NIL(qSH1)与日本晴。其中NIL(qSH1)是携带种子落粒基因qSH1的近等基因系（nearly isogenic line, NIL），该品系种子易落粒，而日本晴无此特性。研究分别选取了NIL(qSH1)和日本晴的幼花不同发育阶段样本，共分为四个采样区域：1. NIL(qSH1)的离层区域；2. NIL(qSH1)离层上方区域；3. NIL(qSH1)离层下方区域；4. 日本晴的离层区域。样本对比实验包含三组：NIL(qSH1)离层与日本晴离层对比、NIL(qSH1)离层与NIL(qSH1)离层上方区域对比，以及NIL(qSH1)离层与NIL(qSH1)离层下方区域对比。