Expression data from HTR-8/Svneo side population (SP) and non-side population (NSP) cells

Immature cell populations, including stem cells and progenitor cells, can be found in “side-population (SP)” cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP HTR-8/SVneo SP cells or NSP cells were isolated using a FACS Vantage fluorescence-activated cell sorter (BD Bioscience, San Jose, California) using dual wavelength analysis (blue, 424 - 444nm; red 675 nm) after excitation with 350 nm UV light. PI-positive dead cells were excluded from the analysis. Sorted SP and NSP cells' RNA had been analyzed by microarray analysis.

包括干细胞与祖细胞在内的未成熟细胞群，可于侧群细胞（side-population, SP）中被检出。尽管已有文献报道从部分成体组织中分离得到SP细胞，但人滋养层SP细胞的分离与鉴定工作仍有待公开报道。本研究借助基因芯片技术，详尽解析了细胞分化背后的全局基因表达程序，并鉴定出HTR-8/SVneo细胞SP亚群中的上调表达基因。实验中，我们使用FACS Vantage型荧光激活细胞分选仪（BD Bioscience，加利福尼亚州圣何塞市），以350nm紫外光激发，通过双波长分析（蓝光波段：424~444nm；红光波段：675nm）分离得到SP细胞与非侧群细胞（non-side-population, NSP）；分析过程中剔除了碘化丙啶（propidium iodide, PI）染色阳性的死细胞。随后，我们通过基因芯片分析了分选获得的SP细胞与NSP细胞的RNA样本。