Genome-wide maps of small RNA transcription in young and old mouse oocytes

We report the abundance of different small RNAs in oocytes of young (8 weeks old) and older (9 months old) mouse females Overall design: Pools of 50 oocytes from young and older females were prepared for small RNA seq using the SMARTer® smRNA-Seq kit. The first batch was sequenced paired end, one sample 2X42 and one sample 2X38 bp, the second to fourth batch was sequenced single end 76 bp.

本研究报道了年轻（8周龄）与老龄（9月龄）雌性小鼠卵母细胞中不同小分子RNA（small RNAs）的表达丰度。
实验设计概述：分别从年轻与老龄雌性小鼠体内收集50枚卵母细胞，制备为两个混合样本池，使用SMARTer® smRNA-Seq试剂盒构建用于小分子RNA测序的样本。首批测序采用双端测序模式，两个样本的测序读长分别为2×42 bp与2×38 bp；第二批至第四批测序采用单端测序模式，读长为76 bp。