CTCF-mediated topological boundaries during development foster appropriate gene regulation. Mus musculus

In this experiment, we performed 5C across a 4Mb region surrounding the HoxA region in WT and Δ5|6:7|9 ESCs and MNs Overall design: 2 lines (WT,Δ5|6:7|9)本实验在野生型（WT）与Δ5|6:7|9细胞系的胚胎干细胞（ESCs, Embryonic Stem Cells）及运动神经元（MNs, Motor Neurons）中，针对HoxA基因区域周边4Mb的基因组区段开展了5C染色体构象捕获实验。实验整体设计：共设置2个样本细胞系，分别为野生型与Δ5|6:7|9