Microbial 16S rRNA gene (DNA) and transcripts (cDNA) along a boreal soil-freshwater-estuary continuum

This repository stores the processed files of the 16S rRNA sequencing reads (DNA and cDNA) of the La Romaine project, which were processed through the DADA2 pipeline. Files are '.rds' files and/or '.csv' files readable by the open statistical software R. The project is part of the Industrial Research Chair in Carbon Biogeochemistry in Boreal Aquatic systems (CarBBAS Chair) led by Paul A. del Giorgio. Samples were pooled by plate ID and season to be processed by DADA2. The number before each '*_seqtab.rds' file corresponds to a pool. ID details are in "splitdf_new.rds". Raw sequences can be found on SRA under the Bioproject number: PRJNA693020. Intermediate processing files are stored here. And scripts are available on Github. Files are being uploaded as manuscripts are published. Currently available files: 2015-2017: 16S rRNA gene and transcripts (DNA and cDNA) in spring, summer, autumn (shallow sequencing) Part of the manuscript: "Terrestrial connectivity, upstream aquatic history and seasonality shape bacterial community assembly within a large boreal aquatic network". The ISME Journal. 2021.本仓库存储La Romaine项目的16S rRNA测序读段（涵盖DNA与cDNA）经DADA2流程处理后的结果文件，文件格式为可由开源统计软件R读取的.rds文件与/或.csv文件。本项目隶属于由Paul A. del Giorgio牵头的北方水生系统碳生物地球化学工业研究讲席项目（CarBBAS Chair）。样本按板编号（plate ID）与季节进行合并分组，随后通过DADA2流程完成处理。每个"*_seqtab.rds"文件名前的数字对应一个样本池，详细ID信息可查阅"splitdf_new.rds"文件。原始序列可在SRA（Sequence Read Archive）数据库中通过生物项目编号PRJNA693020获取。本仓库存储中间处理文件，相关脚本可在Github平台获取。文件将随相关手稿发表逐步上传。当前可获取的文件为2015-2017年春季、夏季、秋季的16S rRNA基因及转录本（DNA与cDNA，采用浅测序策略），相关内容隶属于发表于《ISME Journal》2021年的论文《陆地连通性、上游水生历史与季节节律塑造大型北方水生网络内的细菌群落组装》。