SH1038935.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses, SH）。研究人员通过应用一组动态距离阈值（0.5%至3.0%），将国际核苷酸序列数据库中的所有真菌ITS（Internal Transcribed Spacer）序列聚类至近似物种水平。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier, DOI）形式存在的唯一且稳定的名称，其分类学与生态学注释信息则通过分布式、基于网页的第三方注释工作完成验证。通过整合每个物种假说内所有序列的鉴定结果，研究人员会尽可能将该物种假说与对应分类单元名称及其分类层级（如门、纲、目等）相关联。针对每个物种假说，研究人员会选取一条经自动或人工指定的序列作为其代表序列。这些代表序列已公开发布（https://unite.ut.ee/repository.php），可供科学界用于本地序列相似性搜索、高通量测序分析流程等研究场景。随着公开真菌ITS序列数量的持续增长，该系统与配套数据集将自动完成更新。