microRNA profiling in paired human prostate cancer and benign prostate tissue

To determine the altered microRNA expression signature in human prostate cancer compared to benign prostate tissue. To determine the altered mRNA expression signatures upon overexrpession miR-31 in prostate cancer cells. Two condition experiments:1) Total RNA from 21 pairs of prostate cancers and matched benign prostate tissues were collected and processed for microRNA detection. 2) In LNCaP prostate cancer cells, miR-31 was overexpressed and compared to control miR-NC.

本数据集的研究目标包含两项：其一，明确相较于良性前列腺组织，人类前列腺癌中存在异常表达的微小RNA（microRNA）表达谱；其二，明确前列腺癌细胞在过表达miR-31后，发生异常表达的mRNA（messenger RNA）表达谱。实验共设置两组条件对照：1）收集21对前列腺癌组织及其配对良性前列腺组织的总RNA，用于微小RNA检测；2）在LNCaP前列腺癌细胞中过表达miR-31，并以阴性对照miR-NC作为对照进行比较分析。