Comparative LC/MS-based profiling of L-arginine producing, canavanine resistant Corynebacterium glutamicum strain ATCC 21831 and type strain ATCC 13032

Corynebacterium glutamicum strain ATCC 21831 is a producer of L-arginine that was created by random mutagenesis. It is resistant to the arginine structural analogue canavanine. In order to identify potential bottlenecks in the biosynthetic pathway that leads to this industrially important amino acid, relative metabolite abundances of biosynthetic intermediates were determined in comparison to the type strain ATCC 13032. An extract of U13C-labeled biomass was used as internal standard, to correct for different ionization efficiencies. Metabolites were identified using the ALLocator web platform.

谷氨酸棒杆菌（Corynebacterium glutamicum）菌株ATCC 21831是一株通过随机诱变获得的L-精氨酸（L-arginine）生产菌株，其对精氨酸结构类似物刀豆氨酸具有抗性。为了阐明该工业重要氨基酸生物合成途径中的潜在瓶颈，研究人员以模式菌株ATCC 13032为对照，测定了其生物合成中间体的代谢物相对丰度。本研究使用U13C标记的生物质提取物作为内标，以校正不同的电离效率差异。代谢物的鉴定通过ALLocator在线平台完成。