The E3 Ligase CUL3 physically interacts with TANGO10 protein in vivo

List of protein scores for putative TANGO10 interactors from mass spectrometry analysis of FLAG-tagged TANGO10 using either elav-GAL4 or tim-GAL4 at both ZT10 and ZT 22, as determined using Proteome Discoverer software (ThermoFisher). Fly heads from GAL4 heterozygotes were used as controls. The hits from FLAG-tagged twenty-four (TYF) proteomics were also excluded from the list to increase TANGO10-specificity. Proteins listed are those present in at least one elav-GAL4 sample and at least one tim-GAL4 sample but no negative controls.

本数据集为推定TANGO10相互作用蛋白的评分列表，其数据来源于经FLAG标签（FLAG-tag）标记的TANGO10的质谱分析：实验分别采用elav-GAL4与tim-GAL4两种驱动系，在授时时间（Zeitgeber Time, ZT）10及ZT22两个时间点开展检测，分析过程使用赛默飞世尔（ThermoFisher）的Proteome Discoverer软件完成。实验以GAL4杂合子果蝇的头部作为阴性对照样本。为提升TANGO10的结合特异性，本列表同时剔除了FLAG标记的24 kDa TYF蛋白质组学分析得到的命中蛋白。本次收录的蛋白需满足以下条件：至少在一份elav-GAL4样本与一份tim-GAL4样本中被检出，且未在任何阴性对照样本中出现。