Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [hiplex1]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Map the off-target landscape and characterization of the scissile profile of 1491 genomic gRNA-targets (HiPlex1 library) using BreakTag

本研究开发了BreakTag——一种兼具通用性、高度并行性与剪切感知特性的方法，用于Cas9诱导的DNA双链断裂（double strand break, DSBs）的谱学分析，以筛选影响Cas9切割的分子决定因子。实验整体设计：借助BreakTag绘制1491个基因组gRNA靶标（HiPlex1文库）的脱靶图谱，并对其剪切特征谱进行表征。