In vitro transcriptomic comparison of daptomycin-resistant Staphylococcus aureus

Mutants from SG511 and NCTC8325 (wild type isolates) resistant to daptomycin were obtained following serial passage experiments in vitro. Transcriptomic experiments showed alteration in the expression of genes involved in various pathways including general metabolism, cell wall regulon and lipid metabolism. Microarray was used to evaluate alteration in the transcriptome between wild type strains SG511 and NCTC8325 and their daptomycin resistant isolates, in antibiotic-free medium.

通过体外连续传代实验，成功获取了源自野生型分离株SG511与NCTC8325的达托霉素（daptomycin）耐药突变株。转录组实验结果表明，涉及基础代谢、细胞壁调控子与脂质代谢等多条通路的基因表达均出现改变。本研究采用基因芯片（Microarray）技术，在无抗生素培养基中，对比分析野生型菌株SG511、NCTC8325及其达托霉素耐药突变株之间的转录组差异。