Allopregnanolone alters the gene expression profile of human glioblastoma cells

Glioblastomas (GBM) are one of the most frequent and aggressive brain tumors. In these malignancies, progesterone (P4) promotes proliferation, migration, and invasion. The P4 metabolite allopregnanolone (3α-THP) similarly promotes cell proliferation in the U87 human GBM cell line. Here, we evaluated global changes in gene expression of U87 cells treated with 3α-THP, P4, and the 5α-reductase inhibitor, finasteride (F). Human glioblastoma U87 cell line (purchased from ATCC) was treated with 3α-THP (10 nM), P4 (10 nM), F (100 nM) or vehicle (V, 0.1 %DMSO) during 72 h. Three independent experiments were performed. Total RNA was extracted and prepared for the samples hybridization with GeneChip Human Gene 1.0 ST arrays (Affymetrix microarrays).

胶质母细胞瘤（Glioblastomas, GBM）是最为常见且侵袭性最强的脑肿瘤之一。在这类恶性肿瘤中，孕酮（progesterone, P4）可促进细胞增殖、迁移与侵袭。孕酮的代谢产物别孕烯醇酮（allopregnanolone, 3α-THP）同样可促进人胶质母细胞瘤U87细胞系的细胞增殖。本研究针对经3α-THP、P4以及5α-还原酶抑制剂非那雄胺（finasteride, F）处理的U87细胞的基因表达全局变化展开评估。人胶质母细胞瘤U87细胞系（购自ATCC）分别经10 nM的3α-THP、10 nM的P4、100 nM的非那雄胺，以及溶剂对照（Vehicle, V，0.1% DMSO）处理72小时。共开展3次独立重复实验。提取各组总RNA并制备杂交样本，采用GeneChip Human Gene 1.0 ST基因芯片（Affymetrix微阵列）完成样本杂交。