Transcriptome analysis of LCM samples

We aim to set up a RNA pipeline for LCM samples to study transcriptome landscape of tumor and normal tissues. RNA was extracted from Lazer capture micro-dissection tissues and cDNA libraries were prepared with SMARTseq2 protocol. Libraries are pooled to be sequenced.EGA study EGAS00001003862本研究旨在构建适配激光捕获显微切割（Laser Capture Microdissection, LCM）样本的RNA分析流程，以解析肿瘤与正常组织的转录组全景。研究人员从激光捕获显微切割组织中提取RNA，采用SMARTseq2方法制备cDNA文库，随后将文库混合并进行测序。本研究隶属于欧洲基因组表型数据库（European Genome-phenome Archive, EGA）编号为EGAS00001003862的研究项目。