SBC LTER: Reef: Microsatellite markers for the giant kelp, Macrocystis pyrifera

These data were published as a technical note: Alberto, F., A. C. Whitmer, N. C.
Coelho, M. Zippay, E. Varela-Alvarez, P. T. Raimondi, D. C. Reed and E. A. Serrão. 2009.
Microsatellite markers for the giant kelp Macroystis pyrifera. Conservation Genetics.
10:1915-1917 doi: 10.1007/s10592-009-9853-9 Abstract: We report the isolation and characterization of 16 microsatellite loci to study
the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by
screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs
defined, 16 amplified clean polymorphic microsatellites and are described. These loci
identified a number of alleles ranging from three to forty (mean= 16.5, and gene diversity
ranging from 0.469 to 0.930 (mean= 0.774). The isolation and characterization of these highly
polymorphic markers will greatly benefit much needed studies on the molecular ecology of this
important macroalga. These data report the isolation and characterization of 16 microsatellite loci to study the
population genetics of the giant kelp, Macrocystis pyrifera. 180 samples of blade tissue were
collected at Carpinteria Reef, Santa Barbara Channel, CA (USA) in July 2006. Sequence data are available from GenBank. These data were published as a Technical Note in
the journal Conservation Genetics in 2009.

本数据集相关数据以技术注记形式发表：Alberto, F.、A. C. Whitmer、N. C. Coelho、M. Zippay、E. Varela-Alvarez、P. T. Raimondi、D. C. Reed 与 E. A. Serrão 于2009年在《Conservation Genetics（保护遗传学）》期刊发表的研究成果，卷期页码为10:1915-1917，DOI: 10.1007/s10592-009-9853-9。

摘要：本研究报道了16个微卫星位点（microsatellite loci）的分离与特征开发，用于解析巨藻（Macrocystis pyrifera）的种群遗传学特征。此类标记通过筛选富集微卫星基序的基因组文库获得。在设计的37对引物中，有16对可稳定扩增得到清晰的多态性微卫星位点，本研究对其进行了详细报道。这些位点可检测到3至40个等位基因（平均为16.5），基因多样性范围为0.469至0.930（平均为0.774）。上述高度多态性标记的分离与特征开发，将为这种重要大型藻类的分子生态学相关亟需研究提供重要支撑。

本数据集详细报道了用于巨藻（Macrocystis pyrifera）种群遗传学研究的16个微卫星位点的分离与特征。2006年7月，研究团队于美国加利福尼亚州圣巴巴拉海峡的卡平特里亚礁（Carpinteria Reef）采集了180份叶片组织样本。相关序列数据可从GenBank（基因库）获取。本数据集相关内容于2009年发表于《Conservation Genetics（保护遗传学）》的技术注记栏目。