Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 20mGyT familly).. Mus musculus

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Overall design: Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (20mGyTM), female (20mGyTF) and their progenies (20mGyT1 and 20mGyT2) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线辐照的跨代效应尚未得到充分研究。C57BL/6JNrs雄性小鼠自8周龄起于无辐照饲养环境中饲养400天。本研究通过比对亲代与子代样本，探究新发突变（de novo mutation）情况。本研究属于二次筛选实验，系与日本青森县政府签订合同后实施。整体实验设计为双组对照实验：以C57BL/6JNrs尾部组织非辐照雄性小鼠（20mGyTM）、雌性小鼠（20mGyTF）及其子代（20mGyT1、20mGyT2）为实验组，以参考雄性小鼠为对照组进行比对分析。