PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

The invention relates to a process for the preparation of a fermentation product from lignocellulosic material， comprising the following steps: (a) optionally， pretreatment of the lignocellulosic material; (b) optionally， washing the optionally pretreated lignocellulosic material; (c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO， and optionally purifying the hydrolysed lignocellulosic material; (d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product; and (e) optionally， recovery of a fermentation product， wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material， the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material， preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material， more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material， and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

本发明涉及一种以木质纤维素原料制备发酵产物的工艺，其包含如下步骤：
(a) 木质纤维素原料预处理（可选步骤）；
(b) 对经可选预处理后的木质纤维素原料进行洗涤（可选步骤）；
(c) 使用包含至少两种纤维素酶的酶组合物对经可选洗涤和/或可选预处理的木质纤维素原料进行酶解，该酶组合物至少包含溶解性多糖单加氧酶（LPMO）；并可选地对酶解后的木质纤维素原料进行纯化；
(d) 对酶解后的木质纤维素原料进行发酵以制备发酵产物；
(e) 发酵产物回收（可选步骤）；
其中，工艺过程中需消耗的氧气量为每千克木质纤维素原料中存在的葡聚糖对应20~5000 mmol分子氧；氧气需在预处理后、木质纤维素原料酶解之前和/或酶解过程中加入，优选每千克葡聚糖对应至少30 mmol分子氧的消耗量，更优选至少40 mmol，最优为至少50 mmol分子氧的消耗量。