Additional file 1: Table S1. of Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae

Enterobacteriaceae strains analysed in this study. The deep-branching clade to which they belong (Fig. 1), the presence/absence of fliDCAZ loci, the NCBI accession numbers of the contigs on which these loci occur, and presence/absence of FGI and FMI loci are indicated. Table S2. Characteristics of FGI+ Enterobacteriaceae and their flagellin glycosylation islands. The isolation sources and deep-branching clade to which they belong in the Enterobacteriaceae phylogeny (Fig. 1) are indicated for each of the FGI+ enterobacterial strains. The sizes, G + C contents, G + C deviation from the rest of the genome and number of proteins encoded on each FGI are shown. Table S3. Characteristics of FMI+ Enterobacteriaceae and their flagellin methylation islands. The isolation sources and deep-branching clade to which they belong in the Enterobacteriaceae phylogeny (Fig. 1) are indicated for each of the FMI+ enterobacterial strains. The sizes, G + C contents, G + C deviation from the rest of the genome and number of proteins encoded on each FMI are shown. Table S4. Annotations of the proteins encoded on the enterobacterial FGIs. The number of strains and genera in which orthologs of each distinct protein are encoded within the FGIs are indicated, as well as the closest non-enterobacterial Blast hit, obtained by BlastP analysis against the NCBI non-redundant protein database. Orthologs were only considered among the top 500 BLAST hits and for those orthologs with > 30 % amino acid identity to the query protein. The putative function and conserved domains observed after BLAST analyses against the NCBI protein and conserved domain databases are shown. Table S5. Genomics inserts in the fliDCAZ loci of FGI−/FMI− Enterobacteriaceae. The insert size, G + C content, G + C deviation from the rest of the genome, number of proteins encoded and putative functions of the encoded proteins in each insert are shown. (XLSX 234 kb)

本研究分析的肠杆菌科（Enterobacteriaceae）菌株相关信息如下：其所属的深度分支演化支（图1）、fliDCAZ基因座的有无、这些基因座所在序列重叠群（contigs）的NCBI登录号，以及鞭毛蛋白糖基化岛（Flagellin Glycosylation Island, FGI）和鞭毛蛋白甲基化岛（Flagellin Methylation Island, FMI）基因座的有无均已标注。

表S2 携带FGI的肠杆菌科菌株及其鞭毛蛋白糖基化岛特征。本研究为每株FGI阳性肠杆菌科菌株标注了其分离来源，以及其在肠杆菌科系统发育树（图1）中的深度分支演化支归属；同时列出了每个FGI的序列长度、G+C含量、与基因组其余部分的G+C偏差值，以及该FGI所编码的蛋白质数量。

表S3 携带FMI的肠杆菌科菌株及其鞭毛蛋白甲基化岛特征。本研究为每株FMI阳性肠杆菌科菌株标注了其分离来源，以及其在肠杆菌科系统发育树（图1）中的深度分支演化支归属；同时列出了每个FMI的序列长度、G+C含量、与基因组其余部分的G+C偏差值，以及该FMI所编码的蛋白质数量。

表S4 肠杆菌科FGI所编码蛋白质的注释信息。本研究标注了每个独特蛋白质的同源蛋白在多少菌株和菌属的FGI中被编码，同时列出了通过BlastP分析比对NCBI非冗余蛋白质数据库得到的最近缘非肠杆菌科BLAST同源序列。同源蛋白仅在BLAST前500条命中结果中筛选，且与查询蛋白质的氨基酸一致性需大于30%。此外还列出了通过比对NCBI蛋白质和保守结构域数据库得到的推定功能及所观察到的保守结构域。

表S5 FGI阴性/FMI阴性肠杆菌科菌株的fliDCAZ基因座基因组插入片段特征。本研究列出了每个插入片段的序列长度、G+C含量、与基因组其余部分的G+C偏差值、所编码的蛋白质数量，以及所编码蛋白质的推定功能。（XLSX 234 kb）