Murine IgG (polyclonal) from Listeria Challenged pregnant dams

IgG was collected from virgin and pregnant listeria challenged mouse plasma. Polyclonal IgG was analyzed by reduction, alkylation, and LC-MS with top-down MS/MS (HCD and ETD)to look for glycosylation PTMs

本研究从经李斯特菌攻毒的未交配（处女）小鼠与妊娠小鼠的血浆样本中提取免疫球蛋白G（Immunoglobulin G, IgG）。随后通过还原、烷基化处理，并采用液相色谱-质谱联用（Liquid Chromatography-Mass Spectrometry, LC-MS）结合自上而下质谱/质谱（Top-down MS/MS）技术，辅以高能碰撞解离（Higher-energy Collisional Dissociation, HCD）与电子转移解离（Electron Transfer Dissociation, ETD）两种碎裂模式，对所获得的多克隆免疫球蛋白G进行分析，以筛查其糖基化翻译后修饰（Post-translational Modifications, PTMs）。