Transcriptional profiling of migratory type 2 dendritic cells from draining lymph nodes of WT and STAT6-KO mice.

The signals driving the adaptation of type-2 dendritic cells (DC2s) to diverse peripheral environments remain mostly undefined. We found that differentiation of CD11blow migratory DC2s, a DC2 population unique to the dermis, requires STAT6 and KLF4-dependent IL-13 signaling whereas DC2s in lung and small intestine are STAT6-independent. Overall design: The study contains bulk RNA-seq data and single cell RNA-seq of migratory DC2 sorted from draining lymph nodes of WT or STAT6-KO mice. The bulk RNA-seq data contains 36 samples of migratory XCR1- SIRPa+ DC2 sorted from ear, mesenteric or small intestine draining lymph nodes of uninfected WT or STAT6-KO mice. scRNAseq dataset analysed the transcriptome of 2700 migratory SIRPa+ DC2 from ear draining lymph nodes of WT or STAT6-KO.

介导2型树突状细胞（type-2 dendritic cells, DC2s）适应多样化外周环境的信号机制，目前大多尚未明确。本研究发现，CD11b低表达的迁移性DC2s——一类仅存在于真皮层的DC2亚群——其分化依赖于信号转导与转录激活因子6（signal transducer and activator of transcription 6, STAT6）与Kruppel样因子4（Kruppel-like factor 4, KLF4）调控的白细胞介素13（interleukin 13, IL-13）信号通路；而肺与小肠中的DC2s则不依赖STAT6信号。整体实验设计：本研究包含野生型（wild type, WT）或STAT6基因敲除（STAT6 knockout, STAT6-KO）小鼠引流淋巴结中分选得到的迁移性DC2的批量RNA测序（bulk RNA-seq）与单细胞RNA测序（single-cell RNA-seq, scRNA-seq）数据。其中批量RNA测序共包含36个样本，均来自未感染的野生型或STAT6-KO小鼠的耳、肠系膜或小肠引流淋巴结中分选得到的XCR1⁻ SIRPa⁺迁移性DC2；该单细胞RNA测序数据集则分析了来自野生型或STAT6-KO小鼠耳引流淋巴结的2700个SIRPa⁺迁移性DC2的转录组特征。