Quantification of NiV-F and NiV-G sorting into somatodendritic and axonal domains of rat hippocampal neurons.
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Dendrite to axon (D/A) polarity indexes were calculated as described in Materials and Methods. Values are expressed as mean ± SD (n) (n; number of cells analyzed).
The notation NiV-F-GFP (+NiV-G-HA) refers to the NiV-F-GFP polarity index in cells co-expressing NiV-G-HA. The same applies to the NiV-G-HA (+NiV-F-GFP) notation.
Statistical significance for all groups including NiV-F-GFP and NiV-G-HA constructs was calculated by one-way ANOVA followed by Dunnett's test.
(*)P<0.01 when compared to NiV-F-GFP. Significance between group pairs including Tac or NiV-F-GFP Δ104–109 constructs was calculated by Student's t-test.
(§)P<0.01 when compared to Tac;
(‡)P<0.01 when compared to NiV-F-GFP Δ104–109.
树突-轴突(Dendrite to axon, D/A)极性指数按照材料与方法中描述的方法计算得到。所有数值以均值±标准差(n)表示,其中n为分析的细胞数量。标注形式NiV-F-GFP (+NiV-G-HA) 指代共表达NiV-G-HA的细胞中NiV-F-GFP的极性指数;标注形式NiV-G-HA (+NiV-F-GFP) 的含义与此一致。针对包含NiV-F-GFP与NiV-G-HA构建体的所有组别,统计学显著性通过单因素方差分析(one-way ANOVA)结合Dunnett检验(Dunnett's test)进行计算。(*) 与NiV-F-GFP组相比,P<0.01。针对包含Tac或NiV-F-GFP Δ104–109构建体的组间比较,统计学显著性通过学生t检验(Student's t-test)计算得到。(§) 与Tac组相比,P<0.01;(‡) 与NiV-F-GFP Δ104–109组相比,P<0.01。
创建时间:
2014-05-15



