ChIP-seq analysis of wild-type or PAC1-/- T cells with anti-H3K27-ac. ChIP-seq analysis of wild-type or PAC1-/- T cells with anti-H3K27-ac

To analyze the effects of PAC1 on acetylation of H3K27 in exhausted CD8+ T cells, we isolated CD8+ T cells from spleen of wild-type or PAC1-/- mice post LCMV Cl13 infection and performed ChIP-seq with anti-H3K27-ac. Overall design: Two replicates for each group, including wild-tpe, Pac1-/- and input

为探究PAC1对耗竭型CD8+ T细胞中H3K27乙酰化的调控作用，本研究从经淋巴细胞脉络丛脑膜炎病毒克隆13型（LCMV Cl13）感染后的野生型（wild-type）或PAC1基因敲除（PAC1-/-）小鼠脾脏中分离CD8+ T细胞，并采用抗H3K27-ac抗体进行染色质免疫沉淀测序（ChIP-seq）。实验设计：每组设置两个生物学重复，涵盖野生型组、PAC1基因敲除组以及Input对照组。