Effects of sulpiride on mRNA and protein expression of factors in the YAP-TGF-β-Smad signaling pathway in ARPE-19 cells.

(A) RT-PCR was used to detect the mRNA expression of DRD1, DRD2, YAP, TEAD, and TGF-β2 in ARPE-19 cells, (B)Western blotting was used to detect the protein expression of SMAD7, YAP, TEAD, and TGF-β2 in ARPE-19 cells, (C) Quantitative analysis of DRD1, DRD2, YAP, TEAD and TGF-β2 mRNA expression levels in ARPE-19 cells.(D) quantitative results of protein expression of SMAD7, YAP, TEAD, and TGF-β2 in ARPE-19 cells. (E) Protein expression of TGF-β2 in the supernatant of ARPE-19 cell cultures, determined using ELISA. Data are reported as the means ± SD, n = 3. *p p p (ZIP)

(A) 采用逆转录聚合酶链反应（RT-PCR）检测ARPE-19细胞中DRD1、DRD2、YAP、TEAD及TGF-β2的mRNA表达水平；
(B) 采用蛋白质印迹法（Western blotting）检测ARPE-19细胞中SMAD7、YAP、TEAD及TGF-β2的蛋白表达水平；
(C) 对ARPE-19细胞中DRD1、DRD2、YAP、TEAD及TGF-β2的mRNA表达水平进行定量分析；
(D) 对ARPE-19细胞中SMAD7、YAP、TEAD及TGF-β2的蛋白表达水平进行定量分析；
(E) 采用酶联免疫吸附测定（ELISA）检测ARPE-19细胞培养上清液中的TGF-β2蛋白表达水平。
所有数据以均数±标准差（mean ± SD）表示，n=3。*p p p (ZIP)