Dynamin inhibition affects apoE post translationally.

HMDM were incubated with 30 µM MiTMAB, Dyngo-4a or Dynole-34-2 for 2 h and cellular apoE mRNA levels (A) were determined by real time PCR. CHO-apoE cells (B) were incubated with 30 µM MiTMAB, Dyngo-4a, or Dynole-34-2 for 2 h and secreted apoE levels were determined by ELISA. Data shown in (A) are mean ± SD from triplicate cultures representative of at least 2 individual donors for HMDM while panel B are mean ± SEM from 3 experiments.

将人类单核细胞源性巨噬细胞（HMDM）与30 μM的MiTMAB、Dyngo-4a或Dynole-34-2共同孵育2小时，通过实时聚合酶链式反应（real time PCR）检测细胞内载脂蛋白E（apoE）mRNA水平，结果如图A所示。将转染载脂蛋白E的中国仓鼠卵巢细胞（CHO-apoE cells）与30 μM的MiTMAB、Dyngo-4a或Dynole-34-2共同孵育2小时，通过酶联免疫吸附测定（ELISA）检测其分泌型apoE水平。图A所示数据为三次重复培养的平均值±标准差，实验样本来自至少2名个体供体的HMDM；图B所示数据则为3次独立实验的平均值±标准误。