Comparative analysis of the effects of honey, copaiba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) on second intention healing, in rats.

ABSTRACT Objective: to compare the healing by second intention under the effects of topical application of honey, copaíba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) with a control group in rats. Methods: we carried out a skin resection, 1cm in diameter, on the back of 40 rats allocated to four groups of ten animals. All wounds were cleaned daily with 2ml of 0.9% NaCl solution. The first group (control - GC) was restricted to such procedure. In the wounds of the second (GM), third (GO) and fourth groups (GF), after cleaning, we respectively applied 1ml of honey, 1ml of copaíba oil-resin and 1ml of cream containing fibrinolysin, deoxyribonuclease and chloramphenicol. The wounds were occluded with sterile gauze. Immediately after the incision and on days three, seven and 14 of the experiment, the wounds were copied and contraction was analyzed using planimetry. After euthanasia, we histologically evaluated the inflammatory reaction and collagen in the scars. Results: the reduction of the wound area of GM (p=0.003), GO (p=0.011) and GF (p=0.002) were higher than the GC. The amount of type-I collagen present in GM and GO was higher than in GC and GF groups (p<0.05). There was a predominance of chronic inflammatory stage in GM (p=0.004), GO (p<0.001) and GF (p=0.003) when compared with GC. Conclusion: the topical use of honey and copaíba oil-resin increases wound contraction, the presence of type-I collagen and accelerates healing.

摘要
研究目的：对比蜂蜜、古巴香脂油树脂（copaíba oil-resin）及含纤维蛋白溶酶、脱氧核糖核酸酶与氯霉素的商用外用制剂，与对照组相比对大鼠二期愈合（healing by second intention）的影响。
方法：选取40只大鼠，于背部制备直径1cm的皮肤切除创口，随机分为4组，每组10只。所有创口每日以2ml 0.9%氯化钠溶液清洁。第一组为对照组（control group, GC），仅接受创口清洁操作。第二组（GM）、第三组（GO）及第四组（GF）的创口在清洁后，分别外涂1ml蜂蜜、1ml古巴香脂油树脂及1ml含纤维蛋白溶酶、脱氧核糖核酸酶与氯霉素的乳膏，随后以无菌纱布覆盖创口。分别于术后即刻及实验第3、7、14天对创口进行描记，采用平面测量法（planimetry）分析创口收缩情况。处死后，对瘢痕组织的炎症反应及胶原状态进行组织学评估。
结果：GM组（p=0.003）、GO组（p=0.011）及GF组（p=0.002）的创口面积缩减幅度均显著高于GC组。GM组与GO组的I型胶原含量均高于GC组与GF组（p<0.05）。与GC组相比，GM组（p=0.004）、GO组（p<0.001）及GF组（p=0.003）的炎症反应以慢性炎症阶段为主。
结论：外用蜂蜜与古巴香脂油树脂可增强创口收缩能力，提升I型胶原含量，并加速创口愈合。