DNA methylation profiling of umbilical cord blood cells from Trypanosoma cruzi infected and uninfected mothers. DNA methylation profiling of umbilical cord blood cells from Trypanosoma cruzi infected and uninfected mothers

Genome wide DNA methylation profiling of umbilical cord blood DNA samples using the Illumina Infinium MethylationEPIC array (approximately 850,000 CpGs). Samples included cord blood samples from infants born to women with (exposed) and without (control) infection with Trypanosoma cruzi parasites, to test for a potential epigenetic effect of in utero exposure to maternal infection. Overall design: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip

本数据集采用Illumina Infinium甲基化EPIC芯片（Illumina Infinium MethylationEPIC array）对脐带血DNA样本开展全基因组DNA甲基化谱分析，该芯片可覆盖约850,000个CpG位点。纳入的脐带血样本来自感染克氏锥虫（Trypanosoma cruzi）的暴露组孕妇与未感染的对照组孕妇所娩婴儿，本研究旨在探究宫内暴露于母体感染可能产生的表观遗传效应。实验总体设计如下：对经亚硫酸氢盐转化的DNA进行扩增、片段化处理，随后将其与Illumina Infinium Human MethylationEPIC Beadchip进行杂交。