Expression data of 3 prostate cancer stem cell primary lines comparing spheres and parental/adherent culture. Homo sapiens

Transcriptional profile of PCSC spheres in SCM-1% KO (stem-like cells) vs adherent cultures in PCSC-Celprogen medium (differentiated-like cells) Two-condition experiment: Sphere vs. Parental/adherent cells. Biological replicates: 2 sphere replicates , 2 adherent replicates. Overall design: Early passaged tumor cells were expanded in adherent conditions and then plated in sphere forming conditions (SCM-1KO% medium at low density) to isolate sphere growing cells.

本数据集为两组样本的转录组谱对比实验：一组为SCM-1%基因敲除（SCM-1% KO）环境中培养的PCSC球状体（干细胞样细胞，stem-like cells），另一组为PCSC-Celprogen培养基（PCSC-Celprogen medium）中贴壁培养的分化样细胞（differentiated-like cells）。本实验为双条件对照实验，即球状体细胞与亲本/贴壁细胞的对比。生物学重复设置：球状体细胞组与贴壁细胞组各设2次生物学重复。实验整体设计：将低代次肿瘤细胞先在贴壁培养条件下扩增，随后以低密度接种于SCM-1%基因敲除培养基中进行球状体形成培养，以分离获得球状体增殖细胞。