Gene expression profile of in vitro produced bovine blastocyst with and without Thyroid hormone supplementation. Bos taurus

Bovine IVP blastocysts (day 8) were produced with and without thyroid hormones and gene expression profiles were compared. Overall design: The IVC media (SOF) was supplemented with 50ng/ml of each T3 and T4. Four independent IVF run, each gave 1 control and 1 treated sample. Each sample is a pool of 5 blastocyst. Total Rna was extracted from each sample, amplified, labeled (also with dye swap) and hybridized to the EmbryoGene custom Agilent array (GPL13226). FlexArray software was used for data analysis.

本研究以第8天的牛体外生产（In Vitro Produced, IVP）囊胚为材料，分别设置添加甲状腺激素的处理组与不添加甲状腺激素的对照组，对二者的基因表达谱进行比较分析。
实验设计详情：体外培养（In Vitro Culture, IVC）所用的合成输卵管液（Synthetic Oviductal Fluid, SOF）培养基中，分别添加50ng/ml的三碘甲状腺原氨酸（Triiodothyronine, T3）与甲状腺素（Thyroxine, T4）。共开展4次独立的体外受精（In Vitro Fertilization, IVF）实验，每次实验均获得1个对照组样本与1个处理组样本；每个样本由5枚囊胚混合制备。
提取各组样本的总RNA（Total RNA）后，对其进行扩增、标记（标记过程中同步设置染料互换（dye swap）对照），随后将标记产物杂交至EmbryoGene定制安捷伦（Agilent）芯片（GPL13226）。数据分析采用FlexArray软件完成。