Chromosome-scale, haplotype-resolved genome assembly of a tetraploid potato cultivar

Potato is the most important tuber crop in the world. However, its highly heterozygous and tetraploid genome is a burden for breeding-based improvement, which is reinforced by the lack of haplotype-resolved genome assemblies. Here, we report the 3.1 Gb haplotype-resolved, chromosome-scale assembly of the tetraploid potato cultivar, Ovata, at a haplotyping accuracy of 99.6%, which was achieved by coupling PacBio long read sequencing (HiFi), 700+ single-gamete nuclei sequencing and Hi-C sequencing.

马铃薯是全球最重要的块茎类作物。然而，其高度杂合的四倍体基因组给基于育种的遗传改良带来了极大挑战，而目前缺乏单倍型解析基因组组装（haplotype-resolved genome assemblies）的现状进一步加重了这一困境。本研究报道了四倍体马铃薯栽培品种Ovata的3.1 Gb单倍型解析染色体级基因组组装，其单倍型分型准确率达99.6%；该组装通过整合PacBio长读长测序（HiFi）、700余个单配子细胞核测序以及Hi-C测序技术得以完成。