The miR-23-27-24 Clusters Drive Lipid-Associated Macrophage Proliferation in Obese Adipose Tissue II

Bone marrow isolated from control and cluster KO mice were differentiated to macrophages Macrophages were subsequently metabollically-activated for 24 h to encourage an obese-like ATM phenotype. Cells were harvested for bulk RNA-seq Comparative gene expression profiling analysis of RNA-seq data for control and knockout metabollically-activated bone marrow-derived macrophages (4 biological replicates per genotype; 8 total)

从对照组与簇敲除（cluster KO）小鼠体内分离的骨髓经诱导分化为巨噬细胞。随后将巨噬细胞进行24小时代谢激活处理，以构建肥胖样脂肪组织巨噬细胞（ATM）表型。收集细胞用于批量RNA测序（bulk RNA-seq）。本数据集针对对照组与敲除组代谢激活型骨髓源性巨噬细胞（bone marrow-derived macrophages）的RNA测序数据开展比较基因表达谱分析，每组基因型设置4个生物学重复，总计8个样本。