Human dietary intervention study with blueberry-apple juice. Homo sapiens

Human volunteers (N=143; 98 females and 45 males; aged 18-45 years) consumed one litre of blueberry-apple juice per day for four weeks. Before and after the intervention blood was drawn and lymphocytes were isolated for subsequent RNA isolation. Each participant acted as his own control. Overall design: Each subject acted as his own control, meaning that the fluorescent labeled cRNAs of each subject before and after the intervention were mixed and hybridized on one chip. To rule out any possible dye effect, samples from each subject before and after the intervention were randomly labeled with Cy3 and Cy5.

招募143名人类志愿者（N=143；其中女性98名、男性45名，年龄介于18至45岁之间），每日饮用1升蓝莓苹果汁，持续干预四周。于干预前后采集血液样本，分离淋巴细胞以开展后续RNA提取实验。每位受试者均作为自身对照。总体实验设计：每位受试者均采用自身对照设计，即取同一受试者干预前后的荧光标记互补RNA（cRNA）进行混合，随后在同一张芯片上完成杂交反应。为排除潜在的染料效应，将每位受试者干预前后的样本随机以Cy3与Cy5进行荧光标记。