Data 2. 18srRNA Species Tree Newick
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Phylogenetic species tree. Members of Fabacea (Blue) and Brassicacea (Red), Marchantiaceae (Gray, outgroup). Species are further grouped into Fungal-Associated (Green) and Non-Mycorrhizal (Purple). Based on 18s Small Subunit Ribosomal RNA sequences obtained through keyword search on GenBank and NCBI blastn in species’ genomes. Sequences aligned using MUSCLE through AliView, trimmed to shortest contiguous length, and exported in FASTA format to RAxML-ng. Tree constructed using GTR+G model seed 54321, M.polymorpha manually selected as outgroup.
系统发育物种树。本数据集的类群成员包括豆科(Fabacea,蓝色标注)、十字花科(Brassicacea,红色标注)及地钱科(Marchantiaceae,灰色标注,作为外类群);物种进一步被划分为真菌共生类群(绿色标注)与非菌根类群(紫色标注)。本数据集的序列数据来源于通过GenBank关键词检索,以及从物种基因组中进行NCBI blastn比对获取的18S小亚基核糖体RNA(18S Small Subunit Ribosomal RNA)。序列通过AliView调用MUSCLE软件完成多序列比对,修剪至最短连续序列长度,并以FASTA格式导出至RAxML-ng。系统发育树采用GTR+G进化模型、随机种子54321构建,并手动选取地钱(Marchantia polymorpha,M. polymorpha)作为外类群。



