Table_1_Resveratrol Ameliorates Intestinal Damage Challenged With Deoxynivalenol Through Mitophagy in vitro and in vivo.XLSX

Deoxynivalenol (DON) reduces growth performance and damage intestinal function, and resveratrol (RES) has positive effects on growth performance and intestinal function. The purpose of this study was to investigate the protective mechanism of RES in vitro and vivo challenged with DON. The results showed that dietary supplementation with DON significantly increase the mRNA expression levels of mitophagy- related genes, and protein level for PINK1, Parkin, Beclin-1, Lamp, Atg5, Map1lc, Bnip3, Fundc1, Bcl2l1 and SQSTMS1 (P < 0.05), while supplementation with both RES and DON decreased those indexes in the ileum. Besides DON significantly decreased protein level for Pyruvate Dehydrogenase, Cytochrome c, MFN1, OPA1, and PHB1 (P < 0.05), while supplementation with both RES and DON increased protein level for PHB1, SDHA, and VDAC in the ileum. Moreover, in vitro, we found that DON significantly decreased mitochondrial respiration (P < 0.05), while RES + DON increased the rate of spare respiratory capacity. Also, DON significantly decreased total NAD and ATP (P < 0.05), while RES + DON increased the total NAD and ATP. These results indicate that RES may ameliorates the intestinal damage challenged with deoxynivalenol through mitophagy in weaning piglets.

脱氧雪腐镰刀菌烯醇（Deoxynivalenol, DON）可降低生长性能并损伤肠道功能，而白藜芦醇（resveratrol, RES）对生长性能与肠道功能具有积极调控作用。本研究旨在探究RES在脱氧雪腐镰刀菌烯醇染毒的体内外模型中的保护作用机制。结果显示，日粮添加DON可显著上调线粒体自噬（mitophagy）相关基因的mRNA表达水平，以及PINK1、Parkin、Beclin-1、Lamp、Atg5、Map1lc、Bnip3、Fundc1、Bcl2l1及SQSTM1的蛋白表达水平（P < 0.05）；而同时添加RES与DON则可降低回肠中上述指标的表达量。此外，DON可显著下调丙酮酸脱氢酶（Pyruvate Dehydrogenase）、细胞色素c（Cytochrome c）、MFN1、OPA1及PHB1的蛋白水平（P < 0.05），而同时添加RES与DON则可提升回肠中PHB1、SDHA及VDAC的蛋白水平。体外实验中，我们发现DON可显著降低线粒体呼吸功能（P < 0.05），而RES+DON组则提升了备用呼吸能力比率。同时，DON可显著降低总NAD与ATP含量（P < 0.05），而RES+DON组则可提升总NAD与ATP含量。上述结果表明，RES或可通过调控线粒体自噬，缓解断奶仔猪受脱氧雪腐镰刀菌烯醇攻毒后的肠道损伤。