Next Generation Sequencing Facilitates Quantitative Analysis of Ocular Adnexal B-cell Lymphoma Transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of Ocular Adnexal B-cell Lymphoma Transcriptomes

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to describe NGS-derived transcriptome profiling (RNA-seq) of ocular adnexal B-cell lymphomas (OABLs). Gene expression profiling of RNA was performed on OABL samples to identify pathway alterations and expression changes affected by clinical traits Overall design: mRNA profiles of 31 heterogenous samples from OABL patients were generated by deep sequencing using Illumina Novaseq 6000 according to the manufacturer's direction. RNA was extracted from cryopreserved lymphoma tumor specimens. The sequence reads that passed quality filters were analyzed at the transcript isoform level.

下一代测序（next-generation sequencing，NGS）已革命性地革新了细胞通路的系统层面分析手段。本研究旨在描述基于下一代测序的眼附属器B细胞淋巴瘤（ocular adnexal B-cell lymphomas，OABLs）转录组测序（RNA-seq）数据。研究针对眼附属器B细胞淋巴瘤样本开展RNA基因表达谱分析，以鉴定通路异常及受临床特征影响的表达变化。总体实验设计：本研究采用Illumina NovaSeq 6000测序平台，严格依照厂商操作指南，对31例眼附属器B细胞淋巴瘤患者的异质性样本进行深度测序，以获取其mRNA表达谱。所有RNA均提取自低温保存的淋巴瘤肿瘤组织标本。通过质量过滤的测序读段将在转录本亚型水平开展后续分析。