Short- and long-read RNA-seq of mouse neural stem cells across five developmental stages. Mus musculus

Mouse neural stem cells (NSC) across five developmental stages (E15.5, E17.5, P1.5, P8 and Adult) were collected via fluorescence-activated cell sorting with CD133 and Blbp-GFP as NSC markers. The harvested NSCs were subjected to SMART-seq2 protocol and RNA-seq with both Illumina short-read and Oxford Nanopore long-read sequencing strategies.

本数据集的实验样本为小鼠神经干细胞（neural stem cell, NSC），采集自五个发育阶段：胚胎期15.5天（E15.5）、胚胎期17.5天（E17.5）、出生后1.5天（P1.5）、出生后8天（P8）以及成年期。上述细胞通过以CD133与Blbp-GFP作为神经干细胞标志物的荧光激活细胞分选技术收集获得。所收集的神经干细胞经SMART-seq2实验流程处理后，采用Illumina短读长测序与牛津纳米孔（Oxford Nanopore）长读长测序两种策略开展RNA测序（RNA-seq）。