_A_AgroinfFinalConstructsdCas9EV-GCMS.output.raw

pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMS\output\raw Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in Nicotiana benthamiana to check the expression of the genes.pISA-tree 文件路径：_p_SUSPHIRE_I_T12_CuInducible_S_P1_TransientExp_Nb_A_AgroinfFinalConstructsdCas9EV-GCMSoutput aw 标题：本氏烟草（Nicotiana benthamiana）叶片中铜诱导通路终载体的农杆菌浸润（agroinfiltration）实验 描述：本研究构建了两款载体，均搭载由T11构建的合成启动子（synthetic promoters）调控的蛾类信息素通路3个基因、nptII筛选标记，并分别带有/不带有用于激活的向导RNA1（gRNA1）。与此同时，在pLX载体中构建了另一款载体，其搭载铜诱导型dCas9EV系统，分别带有/不带有向导RNA，可与蛾类信息素通路载体共转化至同一农杆菌（Agrobacterium）菌落中。将同时携带两种质粒（向导/非向导通路 + 非向导/向导型铜诱导dCas9EV系统）的农杆菌，在本氏烟草稳定转化前进行农杆菌浸润，以检测相关基因的表达情况。