ETS1 induction by the omental microenvironment promotes ovarian cancer metastasis [RNA-Seq]. Homo sapiens

ETS1 expression is induced by the microenvironment of ovarian metastasis and this expression allows ETS1 to activate a gene expression program involved in cell invasion and epithelial mesenchymal transision. Overall design: mRNA profiles of luciferase knockdown (negative control) and ETS1 knockodwn in the ovarian cancer cell line HEYA8 were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.

ETS1的表达受卵巢转移微环境诱导，且该表达可介导ETS1激活参与细胞侵袭与上皮间质转化（epithelial-mesenchymal transition）的基因表达程序。实验整体设计：针对卵巢癌细胞系HEYA8，构建荧光素酶敲低（阴性对照）与ETS1敲低的稳定细胞株，通过慢病毒载体介导shRNA稳定表达，并经嘌呤霉素筛选获得阳性克隆；随后采用Illumina HiSeq测序平台对两组样本的mRNA表达谱进行深度测序，每组设置三次生物学重复。