Comparative Transcriptome Analyzes with the Bacilysin producer strain B. subtilis PY79 in Luria Broth Medium. Bacillus subtilis PY79

Bacilysin, formed by L-alanine and L-anticapsin, is also the simplest structured peptide antibiotic known to be synthesized nonribosomally by some strains of Bacillus subtilis. In our previous study, to elucidate the functional role of bacilysin in the producer organism, a genome-wide comparative transcriptome analysis was performed between the wild-type strain PY79, as a natural bacilysin producer, and its bacilysin non-producer derivative strain. The results of this global analysis strongly suggested that bacilysin can be a pleiotropic regulatory factor that affected the expression of various physiological and adaptive cellular processes such as competence development, sporulation, and biofilm formation. As further work, in this project, we aimed to elucidate the direct regulatory role of bacilysin. For this, comparative transcriptome analysis was performed in the presence and absence of bacilysin by next-generation sequencing (RNA-seq) to elucidate the differentially expressed genes in the presence of bacilysin. Subsequently, by performing bioinformatics analysis, the functional role and metabolic activities of the biofilm-related genes were identified, and thereby, the possible regulatory role of bacilysin during the biofilm formation process was elucidated.

由L-丙氨酸（L-alanine）与L-抗荚膜素（L-anticapsin）结合形成的杆菌溶素（Bacilysin）是目前已知结构最简单的肽类抗生素，可由部分枯草芽孢杆菌（Bacillus subtilis）菌株通过非核糖体途径合成。在我们此前的研究中，为阐明杆菌溶素在产菌中的功能作用，以天然产杆菌溶素的野生型菌株PY79及其不产杆菌溶素的衍生菌株为材料，开展了全基因组比较转录组分析。该全局分析结果强烈提示，杆菌溶素可作为一种多效调控因子，影响感受态发育、芽孢形成及生物被膜形成等多种生理与细胞适应性过程的基因表达。作为后续研究工作，本项目旨在阐明杆菌溶素的直接调控作用。为此，我们借助下一代测序（RNA-seq）技术，在添加与不添加杆菌溶素的条件下开展比较转录组分析，以明确杆菌溶素存在时的差异表达基因。随后通过生物信息学分析，鉴定出与生物被膜相关基因的功能及代谢活性，从而阐明杆菌溶素在生物被膜形成过程中潜在的调控作用。